COMPARISON OF THE GENERATION INVITRO OF CHEMOTACTICALLY ACTIVE LTB4 AND ITS OMEGA-METABOLITES BY HUMAN-NEUTROPHILS AND LYMPHOCYTES MONOCYTES

  • 1 July 1987
    • journal article
    • research article
    • Vol. 69  (1) , 221-228
Abstract
To assess the relative contribution of different leucocyte subpopulations to LTB4 production, peripheral blood leucocytes from human donors were separated into polymorphonuclear neutrophils (PMN) and lymphocytes/monocytes (L/M) and were then stimulated in vitro with the Ca-ionophore A 23187 for different times. The supernatants were analysed for their contents of leukotriene B4 (LTB4) and its .omega.-metabolites by HPLC-analysis and column fractions were also examined for their chemotactic activities towards eosinophils in vitro. PMN supernatants contained greater quantities of LTB4, 20-OH-LTB4, 20-COOH-LTB4, and chemotactic activities than did L/M supernatants. On the other hand, the time dependent decrease of LTB4 and chemotactic activity and the increase of .omega.-metabolites were higher in PMN than in L/M. These results would correlate with the greater role of PMN in acute and that of monocytes in chronic inflammation.