Binding of low‐density lipoprotein to monolayer cultures of rat hepatocytes is increased by insulin and decreased by dexamethasone

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Abstract
Rat hepatocytes were maintained in monolayer culture for 20 h in the presence of 10% (v/v) new‐born calf serum and then for a further 1–24 h in serum‐free medium containing 2 g bovine serum albumin/l. The specific binding of human 125‐I‐LDL to two distinct sites was then measured at 4°C. Binding to site 1 was displaced by dextran sulphate while that to site 2 was not. The presence of 1–100 nM insulin for 24 h in the second incubation significantly increased binding to site 1. Significant increases were also seen when cells were incubated with 10 nM insulin for 1 h. No significant effects of insulin on binding to site 2 were observed. In contrast, 10 nM—1 μM dexamethasone decreased binding to both sites. The effects of these hormones were mutually antagonistic.

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