Isolation and Structural Characterization of Sialic Acid-Containing Glycopeptides of the O-GlycosidicType from the Urine of Two Patients with an Hereditary Deliciency in α-N-Acetylgalactosaminidase Activity
- 1 January 1989
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 370 (2) , 661-672
- https://doi.org/10.1515/bchm3.1989.370.2.661
Abstract
Glycopeptides have been isolated from the urine of two patients, aged 5 and 6, with a new lysosomal storage disease characterized by a deficiency in .alpha.-N-acetylgalactosaminidase activity. Isolation of these glycopeptides was achieved using gel filtration and ion-exchange chromatography. Structural determination was done using one- and two-dimensional 500 MHz 1H-NMR spectroscopy and FAB mass spectrometry of native and derivatized glycopeptides.This publication has 16 references indexed in Scilit:
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