Surface modification with protein A for uniform binding of monoclonal antibodies.
Open Access
- 1 August 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 33 (8) , 1338-1342
- https://doi.org/10.1093/clinchem/33.8.1338
Abstract
We describe optimal conditions for immobilization of two monoclonal antibodies to progesterone for solid-phase assays. Polystyrene surfaces are refined with Protein A to achieve uniform, reproducible, stable, and sterically accessible immobilization of immunoglobulins (IgG). To this end, we optimized the amount of immobilized Protein A, the pH of the medium for immobilization, the concentration of antibody, and the polystyrene surface. We also investigated three carriers for solid-phase assays: 12 X 75 mm polystyrene test tubes, Macrowells (Skatron, Inc.; suitable for processing with multiple pipettors), and microwell strips (Immulon II, Dynatech Inc.). Immunoglobulin does not appreciably dissociate from any of these solid matrices, even if the assay procedure takes several hours. Therefore, we postulate that more than one molecule of immobilized Protein A binds to IgG, or that there is an additional interaction between the antibody and the polymer surface.This publication has 1 reference indexed in Scilit:
- Structural Studies on the Four Repetitive Fc-Binding Regions in Protein A from Staphylococcus aureusEuropean Journal of Biochemistry, 1977