Synergism of interleukin‐12 and interleukin‐3 on development of hematopoietic progenitors

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Abstract
The recently cloned cytotoxic lymphocyte maturation factor [CLMF] also called NK cell stimulatory factor [NKSF] or interleukin‐12 [IL‐12] has been described as a growth factor for mature lymphoid cells. The present study investigated whether purified recombinant human IL‐12 could stimulate CFU colony growth. Source of progenitor cells were peripheral blood cells depleted of adherent, CD2‐ and CD56‐positive cells. RhIL‐12 was investigated either alone or in combination with rhIL‐3, rhIL‐6 and rhGM‐CSF. RhIL‐12 alone did not support colony formation of myeloid or erythroid progenitors. RhIL‐12 in combination with rhIL‐3 increased the numbers of BFU‐E and CFU‐GM. No synergism or additive effect was seen with the combination of rhIL‐12 and rhGM‐CSF or rhIL‐12 and rhIL‐6. An additive increase in the number of granulocytic colonies was observed when rhIL‐3, rhIL‐6 and rhGM‐CSF were used together with rhIL‐12. Our result therefore suggest that, in addition to being a potent lymphopoietic stimulator, IL‐12 acts synergistically with IL‐3 in enhancing the sensitivity of hemopoietic progenitors to IL‐3.

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