Structure and Function of 5S Ribosomal Ribonucleic Acid from Torulopsis utilisII. Partial Digestion with Ribonucleases and Derivation of the Complete Sequence
Several large oligonucleotide fragments were obtained by partial digestion of Torulopsis utilis 5S RNA with ribonucleases A [EC 3.1.4.22], T1 [EC 3.1.4.8], and U2. From the results of sequence analysis of these fragments and those described in the preceding paper, the complete nucleotide sequence of T. utilis 5S RNA was determined as follows: (p)pG-G-U-U-G-C-G-G-C-C-A-U-A-U-C-U-A-G-C-A-G-A-A-A-G-C-A-C-C-G-U-U-C-U-C-C-G-U-C-C-G-A-U-C-A-A-C-U-G-U-A-G-U-U-A-A-G-C-U-G-C-U-A-A-G-A-G-C-C-U-G-A-U-C-G-A-G-U-A-G-U-G-U-A-G-U-G-G-G-U-G-A-C-C-A-U-A-C-G-C-G-A-A-A-C-U-C-A-G-G-U-G-C-U-G-C-A-A-U-C-Uoh. A possible model for the secondary structure of this RNA is proposed and discussed in relation to the biological function of 5S RNA in ribosomes.