On the promoter complex formation rate of E.coli RNA polymerases with T7 phage DNA
Open Access
- 25 March 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (6) , 1391-1404
- https://doi.org/10.1093/nar/8.6.1391
Abstract
Influence of ionic strength on the kinetics of the promoter complex formation between E. coli RNA polymerase and T7 phage DNA was investigated using a membrane filter assay. The enzyme-promoter association rate constant was determined. It varies from 109 to 3.107 M−1 sec−1 when the ionic strength is changed from zero to 0.15 M NaCl. Basing on the theoretical analysis of experimental data obtained the model for the promoter site selection assuming the enzyme sliding along the DNA is discussed.Keywords
This publication has 22 references indexed in Scilit:
- Theoretical aspects of DNA-protein interactions: Co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous latticePublished by Elsevier ,2004
- A study of the conditions of the first phosphodiester bond formation by E. coli RNA polymeraseFEBS Letters, 1978
- Nonspecific interactions of Escherichia coli RNA polymerase with native and denatured DNA: differences in the binding behavior of core and holoenzymeBiochemistry, 1978
- High resolution physical mapping of specific binding sites of Escherichia coli RNA polymerase on the DNA of bacteriophage T7Journal of Molecular Biology, 1978
- Interaction of RNA Polymerase with Promoters from Bacteriophage fdEuropean Journal of Biochemistry, 1977
- Studies of the binding of Escherichia coli RNA polymerase to DNA: II. The kinetics of the binding reactionJournal of Molecular Biology, 1972
- Studies of the binding of Escherichia coli RNA polymerase to DNAJournal of Molecular Biology, 1972
- A Restriction enzyme from Hemophilus influenzaeJournal of Molecular Biology, 1970
- Studies of the binding of actinomycin and related compounds to DNAJournal of Molecular Biology, 1968
- The 5′-terminal nucleotides of T7 bacteriophage deoxyribonucleic acidJournal of Molecular Biology, 1966