Characterization and expression of an mRNA encoding a wound-induced (Win) protein from ethylene-treated tomato leaf abscission zone tissue
Open Access
- 1 June 1997
- journal article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 48 (6) , 1223-1227
- https://doi.org/10.1093/jxb/48.6.1223
Abstract
A cDNA clone (TAB7) encoding a putative woundinduced (Win) protein has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) leaf abscission zone cDNA library using a differential screening strategy. The clone has a high degree of homology at the amino acid level to both the potato win1 and 2 genes, Hevea brasiliensis hevein and Nicotiana tabacum PR-4a and PR-4b proteins. The mRNA encoded by TAB7 is up-regulated within 12 h of exposure to ethylene (10μl l−1) and its expression increases steadily within the cells comprising the leaf abscission zone and to a lesser extent in the adjacent non-zone tissue. This rise precedes the onset of cell separation. Southern analysis indicates that the mRNA is encoded by either a single gene or a small gene family. The role of the protein during abscission is discussed.Keywords
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