Heterocaryosis and Somatic Recombination in Cephalosporium mycophilum

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Abstract
Three heterocaryons between auxotrophic strains of Cephalosporium mycophilum have been investigated. In two of the heterocaryons grown on minimal medium, only one of the component nuclear types could be detected in the conidia. Each of the three heterocaryons investigated responded to supplementation of the minimal medium on which they were grown by alteration of nuclear proportions. Analyses of conidia produced by two presumptive somatic diploid colonies have yielded recombinant nuclei bearing markers characteristic of the two parental strains. These investigations suggest the possibility of carrying out genetic analyses in C. mycophilum, a fungus lacking a well defined sexual cycle.