The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues
- 23 November 2004
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 384 (2) , 233-237
- https://doi.org/10.1042/bj20041474
Abstract
The yeast SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) protein Ykt6 was shown to mediate palmitoylation of the fusion factor Vac8 in a reaction essential for the fusion of vacuoles. Here I present evidence that hYkt6 (human Ykt6) has self-palmitoylating activity. Incubation of recombinant hYkt6 with [3H]Pal-CoA ([3H]palmitoyl-CoA) leads to covalent attachment of palmitate to C-terminal cysteine residues. The N-terminal domain of human Ykt6 contains a Pal-CoA binding site and is required for the reaction.Keywords
This publication has 29 references indexed in Scilit:
- Mammalian Ykt6 Is a Neuronal SNARE Targeted to a Specialized Compartment by its Profilin-like Amino Terminal DomainMolecular Biology of the Cell, 2003
- An Autoinhibitory Mechanism for Nonsyntaxin SNARE Proteins Revealed by the Structure of Ykt6pScience, 2001
- Ykt6 Forms a SNARE Complex with Syntaxin 5, GS28, and Bet1 and Participates in a Late Stage in Endoplasmic Reticulum-Golgi TransportJournal of Biological Chemistry, 2001
- New Insights into the Interaction of Ras with the Plasma MembraneCell, 1999
- Membrane Fusion and ExocytosisAnnual Review of Biochemistry, 1999
- Autoacylation of G Protein α SubunitsJournal of Biological Chemistry, 1996
- Protein Sorting by Transport VesiclesScience, 1996
- Biochemical Characterization of a Palmitoyl Acyltransferase Activity That Palmitoylates Myristoylated ProteinsJournal of Biological Chemistry, 1995
- Fatty acylation promotes fusion of transport vesicles with Golgi cisternae.The Journal of cell biology, 1990
- Fatty acyl-coenzyme a is required for budding of transport vesicles from Golgi cisternaeCell, 1989