A Simple Method for Multiple Modification of theEscherichia coliK-12 Chromosome
- 23 December 2007
- journal article
- Published by Oxford University Press (OUP) in Bioscience, Biotechnology, and Biochemistry
- Vol. 71 (12) , 2905-2911
- https://doi.org/10.1271/bbb.70274
Abstract
We developed a simple method of generating markerless deletions in the Escherichia coli chromosome. The method consists of two recombination events stimulated by λ Red recombinase. The first recomb...Keywords
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