Tetracyano-2,2-bipyridineiron(iii), an improved electron acceptor for the spectrophotometric assay of β-oxidation and of succinate dehydrogenase in intact mitochondria

Publisher Website
Google Scholar
Abstract
A recently described direct reading assay for .beta.-oxidation and for succinate oxidation in intact [rat liver] mitochondria using [Fe(CN)6]3- as final electron acceptor was improved by using instead tetracyano-2,2-bipyridineiron(III) [Fe(CN)4(bpy)]-, which gives a 2.6 times greater absorbance change on reduction. Some physical and kinetic properties of [Fe(CN)4(bpy)]- are described. The use of exogenous cytochrome c(III) as electron acceptor was also tested; this gives the largest absorbance change, although the absolute rate of reaction is only .apprx. one-third of that using [Fe(CN)6]3- or [Fe(CN)4(bpy)]-.

This publication has 13 references indexed in Scilit: