Two isoforms of protein phosphatase 1 may be produced from the same gene

Publisher Website
Google Scholar
Abstract
A cDNA clone encoding a second type-1 protein phosphatase catalytic subunit (1α) was isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The deduced protein sequence (330 residues, 37.5 kDa) was 19 residues longer at its N-terminus than protein phosphatase 1β (311 residues, 35.4 kDa). The amino acid sequences of protein phosphatases 1α and 1β were identical after residue 33 of protein phosphatase 1α. The results suggest that the different N-terminal sequences of protein phosphatases 1α and 1β are likely to be generated by differential transcription or processing of the mRNA transcribed from a single gene. Southern blotting of rabbit DNA was consistent with this interpretation.