PURIFICATION OF ANTIBODIES TO INFLUENZA A VIRUS STRUCTURAL PROTEINS BY AFFINITY CHROMATOGRAPHY, AND THEIR PARTICIPATION IN HAEMAGGLUTINATION‐INHIBITION, NEUTRALIZATION AND ENZYME‐LINKED IMMUNOSORBENT ASSAY

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Abstract
Summary: Affinity chromatography was used to purify rabbit antibodies to common and strain‐specific antigeneic determinants of haemagglutinin, to neuraminidase, and to a combination of the internal proteins of influenza A viruses. The purity of the antibodies was assessed by haemagglutination‐inhibition, enzyme‐linked immunosorbent assay (ELISA) and competition ELISA. The antibodies were examined for their participation in neutralization and haemagglutination‐inhibition assays, and in ELISA. ELISA was found to be the most sensitive technique for detecting antibodies. Antibodies to the common and strain‐specific determinants of haemagglutinin were shown to participate in neutralization assays and ELISA, but only those antibodies to the common determinants were detected by haemagglutination‐inhibition.