Measurement of Lactate Extraction Ratio by Centrifugal Analyzer to Assess Myocardial Ischemia

Abstract
Townsend, Ronald M., Gotelli, George, Weingartner, Donys, and Marton, Laurence J.: Measurement of lactate extraction ratio by centrifugal analyzer to assess myocardial ischemia. Am J Clin Pathol 67: 80–83, 1977. The measurement of blood lactate to determine myocardial lactate extraction ratio requires a high degree of within-run precision, since small changes between arterial and coronary sinus lactate may occur. These changes in man may take place at lactate levels in the normal range, 5–18 mg/dl (0.56–2.00 mmol/l). The authors have developed a method for blood lactate determination utilizing commercially available reagents in a centrifugal analyzer (Centrifichem). Within-run precision in the low normal range, 5.4 mg/dl (0.60 inmol/l), showed a coefficient of variation of 7%. Precision extends to 50 mg/dl (5.55 mmol/l), and agreement with blood lactate values obtained with the Dupont ACA is good.

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