Determination of the Kinetic Parameters for the “Suicide Substrate” Inactivation of Bovine Liver Catalase by Hydrogen Peroxide
- 1 January 1996
- journal article
- research article
- Published by Taylor & Francis in Journal of Enzyme Inhibition
- Vol. 10 (3) , 167-175
- https://doi.org/10.3109/14756369609030310
Abstract
The kinetics of the bovine liver catalase inactivation by its suicide substrate, H202 was investigated in sodium phosphate buffer, 50 mM pH 7.0, at 27°C. By combination of the rate equations of two concurrent reactions, decomposition of H202 by catalase and suicide inactivation of catalase by H202 simple, semiempirical kinetic equations were defined and used for the determination of the inactivation rate constant and the partition ratio which were found to be 6.86 ± 0.19 M-1 min-1 and 1.82 × 107± 5.0 × 105, respectively. A close match was found between the experimental data and the equations.Keywords
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