Identification of the Hepatitis C Virus E2 Glycoprotein Binding Site on the Large Extracellular Loop of CD81
Open Access
- 1 November 2002
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 76 (21) , 11143-11147
- https://doi.org/10.1128/jvi.76.21.11143-11147.2002
Abstract
The binding of hepatitis C virus glycoprotein E2 to the large extracellular loop (LEL) of CD81 has been shown to modulate human T-cell and NK cell activity in vitro. Using random mutagenesis of a chimera of maltose-binding protein and LEL residues 113 to 201, we have determined that the E2-binding site on CD81 comprises residues Ile182, Phe186, Asn184, and Leu162. These findings reveal an E2-binding surface of approximately 806 Å2 and potential target sites for the development of small-molecule inhibitors of E2 binding.Keywords
This publication has 24 references indexed in Scilit:
- Binding of Hepatitis C Virus-Like Particles Derived from Infectious Clone H77C to Defined Human Cell LinesJournal of Virology, 2002
- Binding of the Hepatitis C Virus Envelope Protein E2 to CD81 Inhibits Natural Killer Cell FunctionsThe Journal of Experimental Medicine, 2001
- Inhibition of Natural Killer Cells through Engagement of CD81 by the Major Hepatitis C Virus Envelope ProteinThe Journal of Experimental Medicine, 2001
- CD81 extracellular domain 3D structure: insight into the tetraspanin superfamily structural motifsThe EMBO Journal, 2001
- Identification of Amino Acid Residues in CD81 Critical for Interaction with Hepatitis C Virus Envelope Glycoprotein E2Journal of Virology, 2000
- Crystallization of a trimeric human T cell leukemia virus type 1 gp21 ectodomain fragment as a chimera with maltose-binding proteinProtein Science, 1998
- CD81 (TAPA-1): A MOLECULE INVOLVED IN SIGNAL TRANSDUCTION AND CELL ADHESION IN THE IMMUNE SYSTEMAnnual Review of Immunology, 1998
- Establishment of persistent hepatitis C virus infection and replication in vitro.Journal of General Virology, 1997
- The envelope glycoprotein from tick-borne encephalitis virus at 2 Å resolutionNature, 1995
- Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbonsProteins-Structure Function and Bioinformatics, 1991