Metabolic Pattern of Polymorphonuclear Leucocytes Induced by Trypsin-digested Microsomes 1

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Abstract
The addition of trypsin[EC 3.4.21.4]-digested liver microsomes induced cyanide-insensitive respiration in guinea pig polymorphonuclear leucocytes with concomitant acceleration of the hexose monophosphate oxidative pathway. The respiration was insensitive to inhibitors of mitochondrial respiration but sensitive to glycolytic inhibitors. These metabolic alterations are similar to those associated with phagocytosis, though the digested microsomes were apparently not taken up by the cells and probably trigger the metabolic changes by interaction with the cellular membrane. Intact microsomes or microsomes treated with chymotrypsin [EC 3.4.21.1], bacterial pro-teinase, ribonuclease [EC 3.1.4.22], or neuraminidase [EC 3.2.1.18] could not induce such respiration.

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