ACIDIC GLYCOSAMINOGLYCANS INHIBIT THE UPTAKE OF TRITIATED-THYMIDINE BY RABBIT AORTIC SMOOTH-MUSCLE CELLS IN CULTURE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 60  (1) , 34-37
Abstract
[Since in early atherosclerosis there is an increase in sulfated glycosaminoglycan [GAG] and enhanced proliferation of smooth muscle cells], the effect of exogenous GAG on the growth rate of rabbit aortic smooth muscle cells in culture was studied by determination of cell number, cell protein and the uptake of [3H]thymidine as well as its incorporation into DNA. GAG did not affect the growth rate of the cells. Except for heparin all the GAG studied chondroitin 4-sulfate, condroitin 6-sulfate, dermatan sulfate and hyaluronic acid interfered with the uptake of [3H]thymidine into the cells. Errors may arise if [3H]thymidine-methods alone are used when studying the growth rate of cultured cells in the presence of GAG.