Peroxisomal remnants in peroxisome‐deficient mutants of the yeast Hansenula polymorha
- 25 March 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 383 (1-2) , 114-118
- https://doi.org/10.1016/0014-5793(96)00220-7
Abstract
We have analyzed the presence of peroxisomal remnants (‘ghosts’) in three peroxisome-deficient (per) mutants of the yeast Hansenula polymorpha, namely Δper4, Δper5 and Δper10. Under peroxisome-inducing growth conditions peroxisomal membrane proteins (PMPs) were normally synthesized in cells of these mutants. In addition, these cells contained clusters of small membranous vesicles, which were absent in cells grown under peroxisome-repressing growth conditions. These structures displayed typical peroxisomal properties in that they proliferated upon overproduction of Per8p, the H. polymorpha peroxisome proliferation factor. Moreover, in Δper4 and Δper5 these vesicles were susceptible to glucose-induced proteolytic degradation.Keywords
This publication has 18 references indexed in Scilit:
- The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation.The Journal of cell biology, 1995
- The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals.The Journal of cell biology, 1994
- Cloning and characterization of PAS5: a gene required for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris.The Journal of cell biology, 1993
- Protein Import into Peroxisomes and Biogenesis of the OrganelleAnnual Review of Cell Biology, 1993
- Peroxisome biogenesis and function inHansenula polymorphaCell Biochemistry and Function, 1992
- BIOCHEMISTRY OF PEROXISOMESAnnual Review of Biochemistry, 1992
- Peroxisomal Membrane Ghosts in Zellweger Syndrome—Aberrant Organelle AssemblyScience, 1988
- Dihydroxyacetone synthase is localized in the peroxisomal matrix of methanol-grown Hansenula polymorphaArchiv für Mikrobiologie, 1985
- Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polycrylamide to nitrocelluloseJournal of Biochemical and Biophysical Methods, 1984
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976