Thyroid Releasing Hormone Inhibits Testosterone Metabolism by Rat Prostate In Vitro

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Abstract
A method involving thin layer chromatography (TLC) was developed to assess 5 .alpha.-DHT-reductase activity in rat ventral prostate, in terms of the amount of testosterone metabolized to its main 5 .alpha.-reduced compound, dihydrotestoterone (DHT). Using this method an in vitro study was carried out to determine 5 .alpha.-DHT reductase activity is the rat ventral prostate as modulated by thyroid releasing hormone (TRH). The study revealed a decrease in the enzyme activity with increasing doses of TRH, as shown by a decrease in the extent of reduction of testostorone to DHT. The ratios of the counts of DHT and testosterone however did not show any dose related effect.