Covalent Display of Oligosaccharide Arrays in Microtiter Plates
- 24 June 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (28) , 8640-8641
- https://doi.org/10.1021/ja048433f
Abstract
A covalent array for the display of complex oligosaccharides in microtiter plates has been developed. This strategy is conducive to the display of carbohydrates to proteins of interest such as lectins and antibodies, including the broadly neutralizing antibody 2G12 against HIV envelope oligomannose and can be cleaved from the surface for further characterization by mass spectrometry. The system was used to probe the multivalent interaction of 2G12 with an optimal epitope (Kd 0.1 μM).Keywords
This publication has 17 references indexed in Scilit:
- Assembly of sugars on polystyrene plates: a new facile microarray fabrication techniqueTetrahedron Letters, 2004
- Site-Specific Modification of Epstein-Barr Virus-Encoded RNA 1 with N2-Benzylguanosine Limits the Binding Sites Occupied by PKRChemBioChem, 2004
- Antibody Domain Exchange Is an Immunological Solution to Carbohydrate Cluster RecognitionScience, 2003
- Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactionsNature Biotechnology, 2002
- The Chemistry of Amine−Azide Interconversion: Catalytic Diazotransfer and Regioselective Azide ReductionJournal of the American Chemical Society, 2002
- High-resolution Crystal Structures of Erythrina cristagalli Lectin in Complex with Lactose and 2′-α-l-Fucosyllactose and Correlation with Thermodynamic Binding DataJournal of Molecular Biology, 2002
- A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective “Ligation” of Azides and Terminal AlkynesAngewandte Chemie International Edition in English, 2002
- Molecular Recognition of Sialyl Lewisxand Related Saccharides by Two LectinsJournal of the American Chemical Society, 2001
- Minisequencing on oligonucleotide microarrays: comparison of immobilisation chemistriesNucleic Acids Research, 2001
- Toward Automated Synthesis of Oligosaccharides and GlycoproteinsScience, 2001