Purification and properties of α‐1,4‐glucan phosphorylase from the red seaweed Gracilaria sordida (Gracilariales)
- 1 February 1991
- journal article
- Published by Wiley in Physiologia Plantarum
- Vol. 81 (2) , 149-155
- https://doi.org/10.1111/j.1399-3054.1991.tb02122.x
Abstract
α‐1,4‐Glucan phosphorylase (EC 2.4.1.1) from the red seaweed Gracilaria sordida(Harv.) W. Nelson was adsorbed onto starch‐Sepharose 6B and Sephacryl S‐300 under specified conditions. The algal enzyme was purified to homogeneity by these two steps. A molecular weight of 97.4 kDa was observed on SDS‐polyacrylamide gel electrophoresis under reducing conditions, while the native molecular weight was 240 kDa asrevealed by 8‐25% native gradient gel electrophoresis or 245 kDa by gel filtration. The pI of the enzyme was 5.4. It had a Km of 227, 264, 285, and 453 μg ml‐1, respectively, towards glycogen, amylopectin, amylose, and maltodextrin. The enzyme activity was inhibited by cyclohexaamylose, ADP‐glucose, and UDP‐glucose. In contrast to other plant sources, cell‐free extracts of G. sordida contained only one form of phosphorylase.Keywords
This publication has 25 references indexed in Scilit:
- Plant Phosphorylases: Structure and FunctionPublished by Springer Nature ,1983
- Properties of α-glucan phosphorylase from pea chloroplastsPhytochemistry, 1983
- AMYLOSE IN FLORIDEAN STARCHNew Phytologist, 1981
- The Structures and Related Functions of Phosphorylase aAnnual Review of Biochemistry, 1980
- Multiple forms of starch phosphorylase from banana leavesPhytochemistry, 1977
- Starch phosphorylase enzymes in developing and germinating pea seedsPhytochemistry, 1976
- Affinity chromatography studies of the de novo glucan synthesizing phosphorylase isozyme of blue-green algaePlant Science Letters, 1975
- Sweet corn phosphorylase: Purification and propertiesArchives of Biochemistry and Biophysics, 1973
- Gel filtration, aggregation, and the enzymic activity of glycogen phosphorylaseBiochemistry, 1970
- Reevaluation of the Molecular Weights of Glycogen Phosphorylases a and b Using Sephadex Gel Filtration*Biochemistry, 1967