Immuno-fluorescent studies with hookworms I. Antigenic relationships of ova

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Abstract
The fluorescent-antibody test has been applied to ova of Ancylostoma caninum and Necator americanus, with sera from immunized rabbits. Fluorescence was clearly observed both around the embryonated and unembryonated ova, but their contents did not fluoresce. There was cross reaction between A. caninum and N. americanus. The antisera against these 2 species also reacted against A. braziliense. The ova ofAscaris lumbricoides, Trichuris trichiura and Dipylidium caninum showed no reaction either to A. caninum or N. americanus antisera. A. suum ova obtained from the uterus of gravid females showed a non-specific fluorescence.