High resolution two‐dimensional mapping of tissue‐specific polypeptides in the desert locust, Schistocerca gregaria

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Abstract
High resolution two-dimensional gel electrophoresis (2-DE) using immobilized pH gradients was used to map the tissue-specific polypeptides of the desert locust, Schisiocerca gregaria. Highly specific comprehensive 2-DE reference maps (“master gels”) were developed for the brain, corpus cardiacum, subesophageal ganglion, and hemolymph. The polypeptides were well resolved within the pH 4–7 range in the first dimension and within the 14–94 kDa molecular mass range in the second dimension.