MEDIA FOR ENUMERATING STAPHYLOCOCCUS AUREUS TREATED WITH HYDROGEN PEROXIDE IN MILK1
- 1 March 1966
- journal article
- Published by International Association for Food Protection in Journal of Milk and Food Technology
- Vol. 29 (3) , 77-80
- https://doi.org/10.4315/0022-2747-29.3.77
Abstract
Summary Several media were compared with plate count agar (PCA) for determining numbers of Staphylococcus aureus 196E (ATCC 13565) surviving hydrogen peroxide-catalase treatment in milk at 54.4 C. Time intervals required for 90, 99, 99.9, and. 99.99% destruction of S. aureus were determined from survivor curves obtained with each medium. Statistical analyses were made of the intervals for 90 and 99.9% destruction. The time required for 99.9% destruction was significantly longer when survivors were enumerated on PCA than when enumeration was made on Staphylococcus medium 110 (SM110), Staphylococcus medium 110-Egg Yolk agar (SM110-Ey), tryptic soy agar, or trypticase soy agar (TSA). SM110-Ey was the only medium which inhibited growth of survivors sufficiently to give significantly shorter time for 90% destruction than PCA. Heart infusion agar (HIA) did not differ significantly from PCA in ability to support growth of survivors.Keywords
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