Evaluation of the DNS method for analysing lignocellulosic hydrolysates

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Abstract
The dinitrosalicylic acid (DNS) method gives a rapid and simple estimation of the extent of saccharification by measuring the total amount of reducing sugars in the hydrolysate. However, it is subject to interference by citrate buffer and other substances and by the differing reactivities of the various reducing sugars. These interferences become more apparent when complex substrates such as sugar cane bagasse are employed. The paper also shows how the DNS method can be adapted for use on a Technicon Autoanalyser.