The isolation of complementary strands from a mouse DNA fraction.

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Abstract
Native satellite DNA was isolated from either mouse L cells or mouse liver by CsCl density gradient centrifu-gation in fixed-angle rotors. Upon recentrifugation in alkaline CsCl gradients, the satellite DNA forms 2 bands which are designated H and L strands. Isolated H and L strands give melting profiles characteristic of single-stranded polynucleotides and are complementary to each other in base composition. Their banding positions in neutralized CsCl density gradients and behavior on hydroxyapatite columns are consistent with the view that they represent the individual DNA strands of the satellite duplex. After mixing equimolar quantities of H and L strands, double-stranded structures rapidly reform as is shown by density gradient centrifugation, melting profiles, and behavior on hydroxyapatite columns.