Identification of Hsc70 as an influenza virus matrix protein (M1) binding factor involved in the virus life cycle
Open Access
- 27 September 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (24) , 5785-5790
- https://doi.org/10.1016/j.febslet.2006.09.040
Abstract
Influenza virus matrix protein 1 (M1) has been shown to play a crucial role in the virus replication, assembly and budding. We identified heat shock cognate protein 70 (Hsc70) as a M1 binding protein by immunoprecipitation and MALDI‐TOF MS. The C terminal domain of M1 interacts with Hsc70. We found that Hsc70 does not correlate with the transport of M1 to the nucleus, however, it does inhibit the nuclear export of M1 and NP, thus resulting in the inhibition of viral production. This is the first demonstration that Hsc70 is directly associated with M1 and therefore is required for viral production.Keywords
This publication has 26 references indexed in Scilit:
- Influenza A Viruses with Mutations in the M1 Helix Six Domain Display a Wide Variety of Morphological PhenotypesJournal of Virology, 2005
- Assembly and budding of influenza virusVirus Research, 2004
- Nuclear Translocation of Papillomavirus Minor Capsid Protein L2 Requires Hsc70Journal of Virology, 2004
- Identification of Novel Nuclear Export and Nuclear Localization-related Signals in Human Heat Shock Cognate Protein 70Journal of Biological Chemistry, 2004
- Heat Shock Protein 70 Is Related to Thermal Inhibition of Nuclear Export of the Influenza Virus Ribonucleoprotein ComplexJournal of Virology, 2004
- Identification of Hsp90 as a Stimulatory Host Factor Involved in Influenza Virus RNA SynthesisJournal of Biological Chemistry, 2002
- Antiviral activity of cyclopentenone prostanoidsTrends in Microbiology, 1997
- Structure of a bifunctional membrane-RNA binding protein, influenza virus matrix protein M1Nature Structural & Molecular Biology, 1997
- The Highly Inducible Member of the 70 kDa Family of Heat Shock Proteins Increases Canine Distemper Virus Polymerase ActivityJournal of General Virology, 1996
- Nuclear transport of influenza virus ribonucleoproteins: The viral matrix protein (M1) promotes export and inhibits importCell, 1991