Surface areas of lipid membranes
- 7 March 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (5) , 837-840
- https://doi.org/10.1021/bi00598a014
Abstract
Upon photolysis, alkyl pentacyanocobaltate complexes generate alkyl radicals which react rapidly and specifically with nitroxide radicals and which do not penetrate phospholipid bilayers. By measuring the loss of paramagnetic resonance signal intensity when multilamellar liposomes containing a small amount of spin-labeled lipid are exposed to these radicals, the proportion of lipid on the external surface of liposomes was measured. Liposomes prepared under specified conditions from dimyristoylphosphatidylcholine, dipalmitoylphosphatidylcholine and binary mixtures of dipalmitoylphosphatidylcholine and cholesterol all have the same proportion of external lipid.This publication has 3 references indexed in Scilit:
- Membrane-controlled depletion of complement activity by spin-label-specific IgMProceedings of the National Academy of Sciences, 1977
- Structural and dynamical aspects of membrane immunochemistry using model membranesBiochemistry, 1977
- Lateral hapten mobility and immunochemistry of model membranes.Proceedings of the National Academy of Sciences, 1976