Evaluation of Urine Culture Screening by Light-scatter Photometry

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Abstract
Urine screening for bacteriuria by light-scatter photometry (Autobac®) was evaluated for accuracy and compared with a colony count by the calibrated loop method. Incubation time, inoculum size, precision, and interference of particulate matter were evaluated in an effort to standardize the screening procedure. Results showed that urines could be accurately screened for Enterobacteriaceae by inoculating a single Autobac cuvette chamber with 0.1 or 0.2 ml of urine and determining the voltage change after four hours. A change of ≥0.2 units indicates significant bacteriuria. Decreased accuracy was noted for urines having >105 cfu/ml of Pseudomonas species or gram-positive cocci, possibly because these organisms grow more slowly.

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