Chronic Myelocytic Leukaemia: Cytogenetical Studies on Haemopoietic Colonies and Diffusion Chamber Cultures

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Abstract
In 20 patients with chronic myelocytic leukaemia in the chronic phase or in blast crisis, several in vitro (CFU‐C, BFU‐E) and in vivo (diffusion chamber) culture techniques were used to demonstrate the existence of a still diploid cell clone. In 4 out of 12 patients in the chronic phase, Ph1‐negative metaphases were found in the various cultures for haemopoietic progenitor cells but not in the standard suspension cultures used for cytogenetics. However, all the 8 patients investigated during blast crisis had only Phl‐positive and other abnormal clones, indicating the loss of the diploid clone during progression of the disease.