Development of B cell lineages during a primary anti-hapten immune response.
Open Access
- 15 November 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 145 (10) , 3216-3222
- https://doi.org/10.4049/jimmunol.145.10.3216
Abstract
Cell lineage relationships were examined in large numbers of hybridomas derived from a single mouse at 7 days and from a single mouse at 12 days after a primary immunization. The properties of these developing lineages provide unique insight into their biologic selection and differentiation. The unique VDJ junctions, the characteristics of the somatic mutations, and the nonproductive H chain gene rearrangements observed at day 12 all indicate that the hybridomas were derived from a limited number of progenitor B cells. Clonally related hybridomas were also observed at day 7. The activation of these lineages occurred very early, because somatic variants were strongly selected for within a few days after the primary immunization.This publication has 22 references indexed in Scilit:
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