Fate of 14C-Gibberellic Acid in Senescing On-tree ‘Valencia’ Orange Fruit1

Abstract
Mature green ‘Valencia’ orange (Citrus sinensis (L.) Osbeck) fruit were painted with solutions of 25 ppm 14C-gibberellic acid and harvested from the tree 1, 3, 10, 30, and 100 days after application. Radioactivity recovered from the flavedo after 1 day amounted to 2% of that applied and more than 1% could still be recovered after 100 days. About 50% of the radioactivity partitioned at pH 3.0 into ethylacetate in all flavedo samples (day 1 to 100). Migration of the ethylacetate radioactivity in thin layer chromatography (TLC) indicated that it may still be in the form of gibberellic acid.

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