ANDROGEN-BINDING PROTEIN IN THE SEMINAL PLASMA OF SOME MAMMALIAN SPECIES

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Abstract
Samples of seminal plasma from men, stallions, boars, goats, bulls and rams were examined for 5.alpha.-dihydrotestosterone (5.alpha.-DHT)-binding activity using polyacrylamide gel electrophoresis. Androgen-binding protein was detected in ovine, caprine and bovine seminal plasma, but not in human (n = 3), porcine (n = 3) or equine (n = 3) samples. When present, androgen binding appeared to be saturable since a 100-fold excess of unlabeled 5.alpha.-DHT in the incubation medium and gel ensured the total displacement of the peak of radioactivity. In the seminal plasma of the goat, there was a high concentration of androgen-binding protein [(41 .+-. 9) .times. 10-9 mol/l; results are means (.+-. SEM [standard errof of the mean]) from 3 (n) pools of seminal plasma, 2 containing 3 samples each and the 3rd containing 4. The concentration of androgen-binding protein in bovine seminal plasma was about 10 times lower [(5 .+-. 0.4) .times. 10-9 mol/l, n = 3]. Androgen-binding protein was only demonstrated in the seminal plasma of mammals with sperm counts .gtoreq. 109/ml.

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