Biosynthesis of Crystalline by Rabbit Lens Fiber Cells

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Abstract
Rabbit lenses explanted to medium-199 containing serum continued to synthesize crystallins (.alpha., .beta., .gamma.) for at least 48 h. The synthetic rates for .alpha. and .beta.-crystallin polypeptides were relatively high for the first 18 h of incubation; the synthetic rate then stabilized at a reduced level. A concomitant decrease in .gamma.-crystallin synthesis did not occur. Intravitreal injection of 3H-leucine was performed. The in vivo synthetic rate for .alpha.-crystallin was comparable to the initial 18 h in vitro.