Translocation of mycoplasma-like bodies through sieve pores in plant tissue cultures infected with aster yellows

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Abstract
Ultrathin sections of carrot root explants infected with aster yellows showed extensive invasion of the phloem area by mycoplasma-like bodies (MLB's). MLB's were equally distributed on both sides of the sieve plates. The sieve pores in infected tissue cultures appeared mostly to be open and the apparent plugging sometimes seen was due to fixation artifacts as well as to MLB's. As pleomorphic structures, MLB's translocated with or without distorting their shape, according to their size and the degree of plugging of the sieve pores. Small particles, up to 200 nm diam, appeared to move with little or no distortion, but particles larger than 200 nm diam changed their shape to pass through the sieve pore. Three phases were postulated to explain the translocation of large MLB's.

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