The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of DEAE sephadex A50 beads
- 1 May 1976
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 18 (5) , 659-667
- https://doi.org/10.1002/bit.260180506
Abstract
Methods are described which make possible the production of foot-and-mouth disease (FMD) virus from BHK 21 C13 monolayer cells which have been grown on the surface of serum coated DEAE Sephadex A50 beads. The yield of cells and their susceptibility to infection by FMD virus are equivalent to conventional Roux monolayer systems. The potential for the commercial application of the DEAE Sephadex A50 system is discussed in relation to other unit process monolayer systems and in particular to the system in which cells are cultured in a deep bed of small glass spheres.This publication has 10 references indexed in Scilit:
- The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of glass spheresBiotechnology & Bioengineering, 1976
- Microcarrier Cultures of Animal CellsPublished by Elsevier ,1973
- The supply of oxygen to submerged cultures of BHK 21 cellsBiotechnology & Bioengineering, 1972
- Method for bulk culture of animal cells on plastic filmExperimental Cell Research, 1972
- A multisurface tissue propagator for the mass-scale growth of cell monolayersBiotechnology & Bioengineering, 1968
- Decigram quantities of pure foot-and-mouth disease virus from cell culturesBiotechnology & Bioengineering, 1968
- Growth of Cell-strains and Primary Cells on Micro-carriers in Homogeneous CultureNature, 1967
- Submerged culture of hamster kidney cells in a stainless steel vesselBiotechnology & Bioengineering, 1965
- The Fixation of Complement by Virus-Antibody Complexes: Equivalence and Inhibition in the Reactions of the Viruses of Tomato Bushy Stunt and Foot-and-Mouth Disease with Rabbit and Guinea-Pig AntiseraJournal of General Microbiology, 1964
- A Glass Helix Perfusion Chamber for Massive Growth of Cells in vitro.Experimental Biology and Medicine, 1962