Purification and Partial Characterization of the Gastric Juice Inhibitory Substance from Culture Filtrate of Streptomyea bottropensis F4708

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Abstract
In the course of screening studies on new substances inhibiting gastric juice secretion among metabolites of Streptomyces, Streptomyces bottropensis F4708 produced a new principle in its culture filtrate inhibiting gastric juice secretion. A crude active fraction was obtained as an isoelectric precipitate by adjusting the culture filtrate to pH 3.0 and was then defatted. The lipid-free active fraction was purified by chromatography on hydroxylapatite column and gel filtration on Sephadex G-100 column. The purified preparation was named gastric juice inhibitory substance. From the result of chemical analysis, this substance was considered to be a kind of glycoprotein. At the dose of 1.0 mg/kg (i.p.), this substance markedly decreased gastric juice volume, total acid output and total peptic activity in pylorus-ligated rats. It showed an inhibitory effect on gastric acid secretion stimulated by tetragastrin or histamine in the perfused stomach preparation of rats and inhibited ulceration developed in the forestomach of pylorus-ligated rats. However, antagonism between this substance and acetylcholine was not recognized on the contraction of guinea-pig ileum.