Production of virus-free garlic (Allium sativumL.) and shallot (A. ascalonicumL.) by meristem-tip culture

1 January 1987

journal article
research article
Published by Taylor & Francis in The Journal of Horticultural Science and Biotechnology

Vol. 62 (2) , 211-220
https://doi.org/10.1080/14620316.1987.11515771

Abstract

Summary All garlic cultivars used for crop production in the UK are infected with two or more viruses. ISEM decoration tests have indicated that onion yellow dwarf (OYDV), leek yellow stripe and shallot latent viruses (SLV) infect some cultivars in various combinations with other unidentified poty- or carlaviruses. The shallot cv Noordhollandse Strogele was infected by OYDV, SLV and a second potyvirus. Virus-free plants of eight garlic cultivars and the shallot cv Noordhollandse Strogele were produced by meristem-tip culture. The percentage of virus-free garlic plants regenerated was increased from 25–50% to 85% when infected parent plants were subjected to thermotherapy at 38 °C prior to tissue culture.

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