Factors influencing the growth of normal and malignant cells in fluid culture media

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Abstract
In a former communication (Ludford, 1933,) attention has been directed to differences in the growth of mouse tumours in plasma and serum. With the aim of elucidating tire significance of these differences, further series of experiments have been carried out, which have brought to light new facts necessitating the revision of in my former conclusions, as well as those of previous investigators who have worked with fluid media, At the same time further insight has been gained into the complexity of the factors which influence the growth of tumours in serum. It has been found that different strains of transplantable tumours exhibit individual differences when grown in serum, and even tumours of the same strain show marked differences from time to time. Also, both. the type of growth and its extent are influenced by the source from which the serum is derived, that is, whether it is homologous or heterologous. In describing these experiments it will be necessary therefore to deal with different strains of tumours separately. Concerning this strain of tumour I am unable to add anything to the account published in my former paper (Ludford, 1933). Neither in mouse serum, nor in rat serum has it been possible yet to obtain good outgrowths of the sarcoma cells. Pure serum and diluted serum; serum, with and without, embryo extract; serum from young and old rats, male and female, both pregnant and non-pregnant; also different types of cover slips have all been tried without influencing the type of growth.

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