Construction in vitro of “Phage-plasmid” chimerae: a new tool to analyse the mechanism of plasmid maintenance
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 169 (1) , 113-116
- https://doi.org/10.1007/bf00267552
Abstract
In this paper, we report the construction in vitro of chimerae between lambdoid replacement vectors (Murray et al., 1977) and the miniF Apr plasmid: pSC138 (Timmis et al., 1975). λF recombinants were shown to be chimerae between the λ and the F replicons. By genetical tests, we have demonstrated that both λ and F replication mechanisms are functional: the λF recombinant behaves as a non defective plaque forming phage on λ sensitive bacteria and establishes itself as a stable plasmid on recA F- homoimmune bacteria. In the extra-chromosomal state, the λF recombinant apparently retains the controlled autonomous replication and the FI incompatibility characteristics of the F plasmid. The potential experimental uses of these phages are discussed.This publication has 12 references indexed in Scilit:
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