Studies in vitro of the passage of serum proteins across the intestinal wall of young rats

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Abstract
Everted ileal sacs of 18-day-old rats were used as in vitro preparations, and a modified technique for their preparation is described. These sacs remained viable and functional for at least 1 h when incubated at 37 degrees C under aerobic conditions. Such preparations showed transport across the intestine against concentration gradients of L-histidine or of radioactive iodine and oxygen uptake was consistent over the period of the experiment. Homologous and heterologous agglutinins and $^{131}$I-labelled $\gamma $-globulins were transported from the mucosal to the serosal fluid. Gamma-globulins were transmitted from the mucosal to the serosal fluid differentially depending upon the species of origin. A part of the protein absorbed was broken down during transport and an increase in oxygen consumption of the tissues occurred during protein transport.