Immunoferritin and Immunofluorescent Studies With Epstein-Barr Virus and Herpes Simplex Virus by Use of Human Sera and Hyperimmune Rabbit Sera2

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Abstract
Antisera were prepared in rabbits by intravenous immunization with concentrated Epstein-Barr virus (EBV) from Burkitt lymphoma cells. These rabbit antisera and a human EBV-positive serum were labeled with ferritin or fluorescein and tested against EBV-infected cells. The human serum and rabbit sera labeled with ferritin reacted with EBV capsids, but only the human serum labeled with fluorescein reacted with EBV-infected cells. Experiments with herpes simplex virus (HSV) indicated that rabbit anti-HSV sera prepared by intravenous immunization resembled rabbit anti-EBV sera in that both reacted by the immunoferritin method with intranuclear virus capsids but not by the immunofluorescence method with nuclei of virus-infected cells. Immunoferritin studies indicated that EBV and HSV do not share common or cross-reacting capsid surface antigens.