Verticillium wilt of tobacco

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Abstract
Seedlings of Nicotiana tabacum L. cultivars with different levels of resistance to Verticillium dahliae Kleb. were studied to determine how long the pathogen took to infect the plants. The fungus, at high levels of inoculum, entered intact roots and reached the vascular system within 3 days. Conidia introduced into the roots by inoculation were translocated throughout the roots. stem, and midribs within 1 hour, and were lodged progressively more towards the top of the taproot and the base of the stem than the apex. Differences between susceptible and resistant cultivars in the time taken to enter intact roots were not significant, nor were there significant differences in the initial level of infection; but conidial movement was more limited in a field-tolerant cultivar than in the very susceptible or highly resistant ones. The pathogen, after 58 days, had been restricted to a greater degree in the resistant cultivar than in the more susceptible varieties.

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