Comparative Immunology. Active Immunization of Young Alligators with Hemocyanin.

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Abstract
Ten young alligators, Alligator mississippiensis, weighing 900-1440 g were immunized with key-hole limpet hemocyanin (KLH). Blood was obtained and injections made by cardiac puncture. Serums were examined by interfacial titrations and immunoelectrophoresis using KLH as test antigen, and the circulating antibody consists of at least 2 kinds of immunoglobulins that differ in electrophoretic mobility and perhaps size. Results indicate that alligators, in contrast to other reptiles, may possess a primitive type of immunological memory.