Subculture Requirement for Rescue of SV40 from ‘Nonrescuable’ Cell Lines
- 31 December 1978
- journal article
- research article
- Published by S. Karger AG in Intervirology
- Vol. 12 (2) , 89-95
- https://doi.org/10.1159/000149073
Abstract
Virus could be rescued by cell fusion techniques from the H50, BRKSV (Bam I linear), 14B and 14B (1–4) cell lines, which contain the entire SV40 genome but have previously yielded negative results by this method. Virus could not be rescued from the T22 cell line, which was transformed by defective virus. The T-antigen-negative flat revertant cell line, 14B (1–4), converted to T-antigen-positive prior to rescue. Subculture and extensive monitoring for production of infectious virus appeared to be prerequisite for detecting virus rescue.Keywords
This publication has 3 references indexed in Scilit:
- Rescue of infectious virus from permissive monkey cells containing simian virus 40 DNA fragmentsJournal of Virology, 1978
- Isolation and characterization of T antigen-negative revertants from a line of transformed rat cells containing one copy of the SV40 genomeCell, 1978
- The arrangement of simian virus 40 sequences in the DNA of transformed cellsCell, 1976