Competitive ELISA of Chloramphenicol: Influence of Immunoreagent Structure and Application of the Method for the Inspection of Food of Animal Origin
Open Access
- 1 June 2000
- journal article
- research article
- Published by Taylor & Francis in Food and Agricultural Immunology
- Vol. 12 (2) , 115-125
- https://doi.org/10.1080/095401000404067
Abstract
An indirect competitive ELISA for the detection of chloramphenicol (CAP) in food of animal origin (milk, meat, eggs) is described. Influence of immunoreagent structure and composition on the assay sensitivity and specificity was investigated. Two CAP derivatives were used for conjugation with proteins: CAP succinate and a diazo derivative of CAP. Molar incorporation of CAP into the coating conjugates was also varied. To eliminate matrix effect on the assay results, a special casein-containing buffer was used for milk samples, whereas for meat and egg samples a 50-fold dilution of the buffer extracts was needed. The method developed permits CAP concentrations to be determined in the range 0.08100 μg 1−1. The detection limit is 0.08 μg kg−1. Recovery in different food samples averages between 70 and 130%. The method can be applied for inspection of food of animal origin for CAP residues.Keywords
This publication has 10 references indexed in Scilit:
- A Novel immunofluorescence capillary electrophoresis assay system for the determination of chloramphenicol in milkFood and Agricultural Immunology, 1994
- Automated high-performance liquid chromatographic determination of chloramphenicol in milk and swine muscle tissue using on-line immunoaffinity sample clean-upJournal of Chromatography B: Biomedical Sciences and Applications, 1992
- Monitoring of chloramphenicol residues in muscle tissues by an immunoassay (La Carte®test)Veterinary Quarterly, 1990
- Method for the isolation and liquid chromatographic determination of chloramphenicol in milkJournal of Agricultural and Food Chemistry, 1990
- A Sensitive Streptavidin‐Biotin Enzyme‐linked Immunosorbent Assay for Rapid Screening of Residues of Chloramphenicol in MilkFood and Agricultural Immunology, 1990
- Monitoring milk for chloramphenicol residues by an immunoassay (Quik‐card®)Veterinary Quarterly, 1988
- Determination of chloramphenicol in swine muscle tissue using a monoclonal antibody-mediated clean-up procedureJournal of Chromatography A, 1987
- Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assayJournal of Immunological Methods, 1985
- Detection and quantitation of chloramphenicol by competitive enzyme-linked immunoassayAntimicrobial Agents and Chemotherapy, 1984
- Determination of free amino groups in proteins by trinitrobenzenesulfonic acidAnalytical Biochemistry, 1966