Quantitative Histochemistry: Histologic Sampling of Sections of Frozen-Dried Tissues

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Abstract
Microchemical techniques are available for analyses of many enzymes and other components of tissue for which stains are not available. This paper describes techniques of freeze drying and histological sampling of certain cell groups or structures free from surrounding stroma, fat and other types of cells. Quantitative data can be obtained for many enzymes in 0.5 to 5 [mu]g samples of freeze-dried tissue. For example, glucose-6-phosphate dehydrogenase activity was measured and compared in the breast alveoli of mice in estrus, diestrus, gestation and lactation.

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