Early and late effects of in vitro exposure of preimplantation mouse embryos to trypan blue

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Abstract
Preimplantation 129/SvSl mouse embryos obtained by induced superovulation and cultured from the 4‐cell to blastocyst stage were exposed to various concentrations of trypan blue for 48 h or to the LD50 (0.16%) and LD99 (0.35%) concentrations for various lengths of time. At least 2 ½ h exposure to the latter concentrations was required before significant reduction in blastulation rates (80% in controls) occurred. Embryos that blastulated after 48 h of culture were transplanted to adult nulliparous 2‐day‐pseudopregnant 129/SvSl females and fetuses were examined 15 days later. Approximately 70% of implanting control blastocysts developed into fetuses. Significantly fewer fetuses developed after 48 h preimplantation exposure to concentrations of 0.08 % or more of trypan blue. The percentage of resorptions increased with exposure to increasing concentrations of trypan blue during the preimplantation period. The only abnormality found in treated fetuses was subdermal hematoma.